Combined with high throughput metabolomics workflow, the deep 96-well plate-based blood storage and analysis platform can greatly broaden the scope and increase the pace of investigations and development of novel ASs optimized for DEHP-free RBC storage. This approach has the potential to hasten the development of a novel AS with significantly enhanced storage characteristics that can overcome high commercialization hurdles that have prevented the adoption of previous novel ASs. Data availability statement

Corning® 96-well Clear Flat Bottom Polystyrene TC-treated Microplate, Individually Wrapped, with Low Evaporation Lid, Sterile

Conclusion

Nguyen BNB, Chetta J, Shah SB. A novel technology for simultaneous tensile loading and high-resolution imaging of cells. Cell Mol Bioeng. 2012;5: 504–513. Corning® 96-well EIA/RIA Clear Flat Bottom Polystyrene Not Treated Microplate, Individually Wrapped, without Lid, Nonsterile

Corning® 96 Half Area Well Clear Flat Bottom TC-treated Microplate, Individually Wrapped, with Lid, Sterile Kaunas R, Nguyen P, Usami S, Chien S. Cooperative effects of Rho and mechanical stretch on stress fiber organization. Proc Natl Acad Sci U S A. 2005;102: 15895–15900. pmid:16247009Geiger B, Spatz JP, Bershadsky AD. Environmental sensing through focal adhesions. Nat Rev Mol Cell Biol. 2009;10: 21–33. pmid:19197329 Results: Final ATP and hemolysis values for the plate-stored RBCs were comparable to the typical values observed for 6-week storage of leukoreduced AS-3 RBCs in PVC bags under both N and H conditions. Hemolysis was below FDA and EU benchmarks of 1% and 0.8%, respectively, and excluding DEHP from plates during storage, resulted in an inconsequential increase when compared to bag samples. There was a significant difference in ATP levels between the H and N ( Table 1B and Supplementary Figure) samples stored under the respective conditions as previously observed in bag-based studies ( Yoshida and Shevkoplyas, 2010; D'Alessandro et al., 2020). The presence of this significant difference demonstrated the translatability of this platform for both N and H storage conditions. Overall, based on the two metrics of e-hemolysis and ATP, it can be concluded that the novel storage platform is sufficiently equivalent to the standard bag method for RBC storage for the purpose of initial AS screening and usable under both H and N storage conditions.